In order to get an accurate measurement, you need to repeat the process for Anne's lower slice (image 5/9). Here are the steps you should follow:
(1) Trace the outline of the lungs,
(2) measure the area of that polygon (don't click elsewhere in the image, or you'll have to re-draw the polygon!),
(3) threshold the polygon,
(4) measure the thresholded area, and
(5) record your answers.
Anne's total lung area (TLA) is the sum of the upper lung area and the lower lung area. Her total dead air (TDA) is the sum of the areas of dead air in her upper and lower slices.